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Literature summary extracted from

  • Riley, J.G.; Menggad, M.; Montoya-Peleaz, P.J.; Szarek, W.A.; Marolda, C.L.; Valvano, M.A.; Schutzbach, J.S.; Brockhausen, I.
    The wbbD gene of E. coli strain VW187 (O7:K1) encodes a UDP-Gal: GlcNAcalpha-pyrophosphate-R beta1,3-galactosyltransferase involved in the biosynthesis of O7-specific lipopolysaccharide (2005), Glycobiology, 15, 605-613.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.303 additional information detergents in the assay do not increase glycosyl transfer Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.303 expressed as a GST-fusion protein encoded by pCM227 in DH5alpha Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.303 CHAPS activity is diminished in the presence of CHAPS at concentrations of 0.125–0.6% Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.303 0.08
-
 1-O-(11-phenoxyundecyl-diphosphoryl)-alpha-D-glucopyranoside pH 7.0, 37°C Escherichia coli
2.4.1.303 1.2
-
 UDP-alpha-D-galactose pH 7.0, 37°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.303 Co2+ 5 mM, yields 38% activity, respectively, compared with 5 mM MnCl2 Escherichia coli
2.4.1.303 Mg2+ 5 mM, yields 11% activity, respectively, compared with 5 mM MnCl2 Escherichia coli
2.4.1.303 Mn2+ optimal activity in presence of 2.5 mM Mn2+. Without addition of divalent metal the activity is less than 4.5% of the activity in presence of Mn2+ Escherichia coli
2.4.1.303 Zn2+ 5 mM, yields 9% activity, respectively, compared with 5 mM MnCl2 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.303 Escherichia coli
-
-
-
2.4.1.303 Escherichia coli VW187
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.303
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.303 UDP-alpha-D-galactose + 1-O-(11-phenoxyundecyl-diphosphoryl)-alpha-D-glucopyranoside synthetic substrate, which is an analog of N-acetyl-D-glucosaminyldiphospho-ditrans,octacis-undecaprenol Escherichia coli UDP + 1-O-(11-phenoxyundecyl-diphosphoryl)-3-O-beta-D-galactopyranosyl-alpha-D-glucopyranoside
-
 ?
2.4.1.303 UDP-alpha-D-galactose + 1-O-(11-phenoxyundecyl-diphosphoryl)-alpha-D-glucopyranoside synthetic substrate, which is an analog of N-acetyl-D-glucosaminyldiphospho-ditrans,octacis-undecaprenol Escherichia coli VW187 UDP + 1-O-(11-phenoxyundecyl-diphosphoryl)-3-O-beta-D-galactopyranosyl-alpha-D-glucopyranoside
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.303 UDP-Gal:GlcNAcalpha-pyrophosphate-R beta1,3-galactosyltransferase
-
 Escherichia coli
2.4.1.303 WbbD
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.303 6 8 broad optimum Escherichia coli

General Information

EC Number General Information Comment Organism
2.4.1.303 malfunction a mutant strain with a defective wbbD gene is unable to form O7 LPS and lacks this specific galactosyltransferase activity. The normal phenotype is restored by complementing the mutant with the cloned wbbD gene Escherichia coli
2.4.1.303 physiological function the enzyme is involved in the biosynthesis of the O7-specific lipopolysaccharide Escherichia coli